Cattle Fetal Hemoglobins by Isoelectric focusing on Polyacrylamide Gel
نویسندگان
چکیده
منابع مشابه
Isoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.
Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...
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A preparative isoelectric focusing (IEF) method was applied to separate skim milk proteins using the Rotofor device in a pH 3-10 gradient containing 4 M urealI % triton X-I 00. Each ofthe 20 fractions obtainedfrom the Rotofor device was then analyzed by polyacrylamide gel electrophoresis (PAGE). Both ureaPAGE and SDS-PAGE were used to separate purified caseins and skim milk resulting in compara...
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ژورنال
عنوان ژورنال: Animal blood-group research information
سال: 1983
ISSN: 1884-3867
DOI: 10.5924/abgri1983.1983.34